What is the function of 2 deoxy D-glucose?

What is the function of 2 deoxy D-glucose?

2-Deoxy- d-glucose is a glucose molecule which has the 2-hydroxyl group replaced by hydrogen, so that it cannot undergo further glycolysis. As such; it acts to competitively inhibit the production of glucose-6-phosphate from glucose at the phosphoglucoisomerase level (step 2 of glycolysis).

How do you make 2 deoxy glucose?

The preparation method comprises the following steps: taking 3,4,5-tri-O-acetyl D-glucal or 3,4,5-tri-hydroxy-glucal as a raw material, and is placed in a solvent under existence of solid acid, stirring and reacting for certain time under certain temperature, filtering to remove solid acid, washing the solid acid by …

How does 2-deoxyglucose affect glycolysis?

Acting as a d-glucose mimic, 2-DG inhibits glycolysis due to formation and intracellular accumulation of 2-deoxy-d-glucose-6-phosphate (2-DG6P), inhibiting the function of hexokinase and glucose-6-phosphate isomerase, and inducing cell death.

How would 2-deoxyglucose affect ATP production?

The glucose analog, 2-deoxyglucose (2DG), inhibits glycolysis, and is predicted to decrease the viability of tumor cells, which are primarily dependent on the glycolytic pathway for ATP generation.

Is 2-deoxyglucose a competitive inhibitor?

Although metrizamide, 2-deoxyglucose, and glucosamine are known to be competitive inhibitors of approximately equal potency for glucose of yeast hexokinase (Ki approximately 0.7 mM for all three), metrizamide is a much weaker competitive inhibitor (Ki about 20 mM) of rat brain hexokinase than either 2-deoxyglucose or …

Where does 2-deoxyglucose inhibit glycolysis?

2-Deoxyglucose (2-DG) is a glucose analog that inhibits glycolysis9,10. 2-DG can compete with glucose to bind hexokinase (the first rate-limiting enzyme of glycolysis) in cells and inhibit metabolism of tumor cell, thereby inhibiting cell proliferation11.

Is 2-DG trapped in the cell?

2DG enters the cell through the glucose transporter and is phosphorylated by hexokinase. Due to low levels of intracellular phosphatase, 2-DG-PO4 is trapped in the cell.

What enzyme does 2-DG inhibit?

2-DG, the glucose analog, acts as a competitive inhibitor of the glycolytic enzyme hexokinase26.